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Further analysis revealed that 675 genes were expressed differently after Tubb4b deletion and were enriched in negative regulation of cell population proliferation (GO:0008285), negative regulation of cell cycle G2/M phase transition (GO:1902750), and positive regulation of cell death (GO: 0010942). We detected the mRNA levels of the cell-regulating cyclins CyclinsD1, CyclinsE, Cdk2, Cdk4, P21, Skp2 and the cell growth factors C/EBP α, C/EBP β, and G-CSF in the spermatogonia of Tubb4b-KO and found that the expressions of CyclinsD1, Skp2 and cell growth factors were significantly reduced. Although spermatogonia lacking TUBB4B have abnormal divisions, they are not lethal. Compared to control mouse spermatogonia, the proliferation rate was significantly slower and cycling stagnated in the G1/0 population. Tubb4b-KO spermatogonia recognized abnormal lysosomal membranes and cell morphology defects. To study TUBB4B-related functions in mouse reproductive development, we designed a single sgRNA in chromosome 2 and generated a knockout spermatogonia cell line of the β-tubulin isoform Tubb4b by the CRISPR/Cas9 system.

Previous studies have shown that TUBB4B affects mouse pronucleus migration, but the gene function has yet to be elucidated. Not for use in the treatment or diagnosis of disease.Tubb4b (tubulin β-4b chain) is essential for cell growth and development as a microtubule network protein. Standards-setting activities of the US Pharmacopeia during the 2000–2005 cycle.

ICH Quality Guidelines: An Implementation Guide, 2017: 467. ICH Q6B Specifications: Test Procedures and Acceptance Criteria for Biotechnological/Biological Products. Fast protein sequencing of monoclonal antibody by real-time digestion on emitter during nanoelectrospray//MAbs. Customized Service: You can choose the appropriate test item according to your project, and we will customize a unique solution for you.Ĭreative Proteomics' analytical scientists can provide quick turnover, clear and concise written reports and customized services to help customers solve analytical and technical problems.Rapid Turnaround Time: 5-10 days to provide comprehensive report.Our bioinformatics analysts can help you interpret the final data.

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Protein sequence analysis